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Image Search Results
Journal: Clinical and Experimental Immunology
Article Title: Exacerbation of spontaneous autoimmune nephritis following regulatory T cell depletion in B cell lymphoma 2‐interacting mediator knock‐out mice
doi: 10.1111/cei.12937
Figure Lengend Snippet: B cell lymphoma 2‐interacting mediator (Bim)–/– and Bim+/– mice showed normal kidney function at 2 months of age and normal kidney histology at 6 months of age. (a) Polymerase chain reaction (PCR) analysis of genomic DNA from C57BL/6 wild‐type (WT) (B6), Bim knock‐out (Bim–/–) and Bim heterozygous (Bim+/–) mice. (b) Proteinuria and (c) serum creatinine were measured in Bim–/–, Bim+/– and B6 WT mice for the first 6 months (*P < 0·05). (d) Representative histological sections stained with periodic acid‐Schiff (PAS) in Bim–/–, Bim+/– and B6 WT mice at 2 months of age (five mice in each group, magnification, ×200, P < 0·05). (e) Representative flow cytometry for CD4+CD25+ T cells and CD4+forkhead box protein 3 (FoxP3+) T cells from splenocytes in Bim–/–, Bim+/– and B6 WT mice at 2 months of age (data are representative of three independent experiments, n = 5 for each group, P < 0·05).
Article Snippet: Genomic DNA was isolated from mouse ear by using
Techniques: Polymerase Chain Reaction, Knock-Out, Staining, Flow Cytometry
Journal: Clinical and Experimental Immunology
Article Title: Exacerbation of spontaneous autoimmune nephritis following regulatory T cell depletion in B cell lymphoma 2‐interacting mediator knock‐out mice
doi: 10.1111/cei.12937
Figure Lengend Snippet: Serum and kidney cytokines were increased significantly after regulatory T cell (Treg) depletion in B cell lymphoma 2‐interacting mediator (Bim)–/– mice. Serum interferon (IFN)‐γ, tumour necrosis factor (TNF)‐α and interleukin (IL)−4 (a), IL‐6, IL‐17a and IL‐2 (b), IL‐10 (c) from four groups of Bim mice were measured at 6 months (data are representative of three independent experiments, n = 5, *P < 0·05 or **P < 0·01). (d) Real‐time quantitative polymerase chain reaction (PCR) for cytokine IL‐6, IL‐10 and transforming growth factor (TGF)‐β mRNA levels in kidneys were evaluated for four groups of mice. Non‐significant changes in IL‐6, IL‐10 and TGF‐β mRNA were found in the kidney of mice in the Bim–/– PC61 group (four mice for each group, each sample was run in triplicate).
Article Snippet: Genomic DNA was isolated from mouse ear by using
Techniques: Real-time Polymerase Chain Reaction